Tandem Mass Spectrometry and Structural Elucidation of Glycopeptides from a Hydroxyproline-rich Plant Cell Wall Glycoprotein Indicate That Contiguous Hydroxyproline Residues Are the Major Sites of Hydroxyproline O-Arabinosylation
نویسندگان
چکیده
منابع مشابه
Contiguous hydroxyproline residues direct hydroxyproline arabinosylation in Nicotiana tabacum.
Hydroxyproline (Hyp) O-glycosylation characterizes the hydroxyproline-rich glycoprotein (HRGP) superfamily of the plant extracellular matrix. Hyp glycosylation occurs in two modes: Arabinosylation adds short oligoarabinosides (Hyp-arabinosides) while galactosylation leads to the addition of larger arabinogalactan polysaccharides (Hyp-polysaccharides). We hypothesize that sequence-dependent glyc...
متن کاملOxygen fixation into hydroxyproline of plant cell wall protein.
The discovery of bound hydroxyproline in higher plants (1, 2) and the demonstration that this hydroxyproline is in peptide linkage (2, 3) and localized in the primary cell wall (3-8) shows that study of the metabolism and role of hydroxyproline can no longer be restricted to the field of collagen studies. Previous work (3-6) indicates that the wall-bound hydroxyproline is derived from free prol...
متن کاملThe cell wall hydroxyproline-rich glycoprotein RSH is essential for normal embryo development in Arabidopsis.
Although a large number of embryo mutants have been studied, mostly at the morphological level, the critical molecular and cellular events responsible for embryogenesis are unknown. Here, we report that using an enhancer-trap embryo mutant of Arabidopsis, we identified a gene, ROOT-SHOOT-HYPOCOTYL-DEFECTIVE (RSH), that is essential for the correct positioning of the cell plate during cytokinesi...
متن کاملA developmentally regulated hydroxyproline-rich glycoprotein from the cell walls of soybean seed coats.
In soybean seeds the level of hydroxyproline is regulated in a developmental and tissue-specific manner. The seed coat contains approximately 77% of the total hydroxyproline in the seed at all stages of development. We determined the ratio of hydroxyproline to dry weight in a number of tissues within the seed; however, only the seed coat shows an increase in this ratio during development. Withi...
متن کاملCell wall regeneration in Chlamydomonas: accumulation of mRNAs encoding cell wall hydroxyproline-rich glycoproteins.
The unicellular alga Chlamydomonas reinhardtii is surrounded by a cell wall composed entirely of hydroxyproline-rich glycoproteins (HRGPs). When the walls of vegetative cells are removed with the enzyme gamete autolysin (g-lysin), they regenerate a matrix within 3-4 hr. In vitro translation of mRNAs isolated from g-lysin-treated cells showed significant increases and decreases in abundance of s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1995
ISSN: 0021-9258
DOI: 10.1074/jbc.270.6.2541